Pacific Biosciences has a Single Molecule Real-Time (SMRT) DNA sequencing, due to be released commercially in 2010 and could enable $100 genome sequencing in 15 minutes in 2013. The second generation real time DNA reader in 2013 is the one that is expected to hit the $100 genome sequencing price. They will release a product in 2010 but it will not be that cheap.
Instead of inspecting DNA copies after polymerase has done its work, SMRT sequencing watches the enzyme in real time as it races along and copies an individual strand stuck to the bottom of a tiny well. Every nucleotide used to make the copy is attached to its own fluorescent molecule that lights up when the nucleotide is incorporated. This light is spotted by a detector that identifies the color and the nucleotide -- A, C, G, or T.
By repeating this process simultaneously in many wells, the technology hopes to bring about a substantial boost in sequencing speed. "When we reach a million separate molecules that we're able to sequence at once … we'll be able to sequence the entire human genome in less than 15 minutes," said Turner.
http://nextbigfuture.com/2009/08/pacific-biosciences-real-time-dna.html
No comments:
Post a Comment